Reconstitution is the process of converting lyophilized (freeze-dried) peptide powder back into an injectable solution using bacteriostatic water (0.9% benzyl alcohol in sterile water for injection). The benzyl alcohol acts as a preservative, allowing multi-dose use over 28-30 days. Standard reconstitution procedure: (1) Allow both vials to reach room temperature. (2) Swab rubber stoppers of both vials with 70% isopropyl alcohol pads and allow to air dry. (3) Using a fresh syringe (typically 3mL for drawing BAC water), draw the calculated volume of BAC water. (4) Insert needle through the peptide vial stopper at a slight angle. (5) Aim the stream down the inside wall of the vial — direct impact on the lyophilized cake can damage peptide structure and reduce potency. (6) Allow the water to slowly dissolve the peptide by gravity and diffusion. Gently swirl in a circular motion if undissolved particles remain after 2-3 minutes. NEVER shake vigorously — this creates foam and can denature the peptide through shearing forces. (7) Once fully dissolved (clear solution, no visible particles), the reconstituted peptide is ready for dosing. (8) Immediately refrigerate at 2-8°C (standard refrigerator temperature).
reconstitution
Reconstitution Basics — How to Mix Peptides
💡 Tips
COMMON MISTAKES: (1) Squirting BAC water directly onto the powder — this can shatter the lyophilized cake and create frothy foam that is hard to dose accurately. (2) Shaking the vial — peptides are delicate proteins; vigorous shaking denatures them. (3) Using sterile water instead of BAC water — sterile water has no preservative and must be used within 24 hours; BAC water lasts 28-30 days. (4) Not refrigerating after reconstitution. (5) Using too little or too much BAC water — this does not change the total peptide amount but affects concentration and dosing convenience. PRO TIPS: Use an online reconstitution calculator (many free ones exist) to determine exact syringe units for your dose. Always label your vials with the peptide name, reconstitution date, and concentration after mixing.
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